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DIFFERENTIATION OF NEUROBLASTOMA NB2A CELLS VIA INVOLVEMENT OF THE CACYBP/SIP ERK1/2 PATHWAY
Supervisor:
Anna Filipek, Ph.D
Supervisor webpage
http://www.nencki.gov.pl/en/working_groups/molecular_and_cellular_neurobiology/lab_01.html
Foreign partner:
Prof. Walter J. Chazin, Ph.D, Departments of Biochemistry and Chemistry, Center for Structural Biolo
Foreign partner webpage:
https://medschool.mc.vanderbilt.edu/biochemistry/php_files/show_partbiochemistry.php?id3=931
Background:
Cell differentiation is regulated by many cofactors, among them are the extracellular signal regulated kinases 1 and 2 (ERK1/2). CacyBP/SIP was originally discovered in our laboratory as a S100A6 (calcyclin) binding partner and is present mainly in brain neurons. Recently, we have showed that CacyBP/SIP is up-regulated during differentiation of neuroblastoma NB2a cells and that CacyBP/SIP interacts with ERK1/2. Moreover, the CacyBP/SIP-ERK1/2 interaction regulates the Elk-1 phosphorylation/transcriptional activity in vitro and in the nuclear fraction of neuroblastoma NB2a cells.
Aim of the study:
The aim is to study the molecular mechanism leading to cell differentiation by involving the CacyBP/SIP-ERK1/2 complex.
International exchange:
During the stay in the foreign laboratory, the Ph.D student will be involved in: preparing plasmids, purification of recombinant proteins, determination of binding constant of CacyBP/SIP to ERK2 and performing the NMR experiments.
Additional requirements for the student:
Experience in protein chemistry, molecular biology, cell biology.


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