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PARTICIPATION OF ANNEXINS IN CALCIUM-DEPENDENT CATECHOLAMINE SECRETION BY PC12 CELLS
Supervisor:
Prof. S³awomir Piku³a, Ph.D
Supervisor webpage
http://www.nencki.gov.pl/en/working_groups/biochemistry/lab_01.html
Foreign partner:
Prof. René Buchet, Ph.D, Université Lyon I, UFR Chimie-Biochimie, Villeurbanne Cedex, France
Foreign partner webpage:
Background:
Upon stimulation neuroendocrine cells secrete catecholamines, noradrenaline and dopamine, that elicit pleiotropic action in the organism. Elevation of [Ca2+]c is a prerequisite for transport of chromaffin granules carrying catecholamines, their fusion with plasma membrane and release of catecholamines to the extracellular milieu. Several calcium binding protein classes, among them annexins (a family of Ca2+/cholesterol-dependent phospholipid-binding proteins), are known to participate in the calcium-regulated exocytosis. In addition, annexins are suggested to play a role in regulation of biological membrane dynamics and are also considered as involved in vesicular trafficking. While recent ex vivo experiments confirmed the participation of AnxA2, AnxA4 and AnxA7 in catecholamine secretion, the role of AnxA6 in this process has not been investigated yet. Due to alternative mRNA splicing AnxA6 is expressed in mammalian tissues as two isoforms, the full-length AnxA6-1 and AnxA6-2 lacking the 524-VAAEIL-529 sequence. So far little is known about differences in physiological functions of these isoforms.
Aim of the study:
The aim of the proposed study will be to dissect the role of AnxA6 and AnxA2 isoforms in Ca2+ homeostasis and their influence on catecholamine secretion.
International exchange:
The PhD student is expected to spend a total of 24 months in Prof. Rene Buchet\'s laboratory where she/he will be trained in the techniques aimed to study the structure of recombinant proteins and their interactions with lipid membranes using various spectroscopic and microscopic techniques.
Additional requirements for the student:
The candidate should have master degree in biological sciences (preferentially cellular and molecular biology, biochemistry and/or biophysics) within 2-3 years before the call. Good English communication skills are required. We are particularly interested in candidates with prior experience in microRNAs.


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